Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.

Description
Rabbit polyclonal antibody to AS250

Applications 
WB, IF, ICC, IHC.

Immunogen 
AS250 Antibody detects endogenous levels of total AS250.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)

Molecular weight
210 KD; 211kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AS250

Synonyms 
250 kDa substrate of Akt; AS250; bA287B20.1; dJ1049G11; dJ1049G11.4; KIAA1272; p220; Ral GTPase-activating protein alpha subunit 2; Ral GTPase-activating protein subunit alpha-2; RALGAPA2; RapGAPalpha2; RGPA2_HUMAN; RP11-470C13.2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q2PPJ7

ab14378 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody....

ab14378 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14378) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue)....