Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.

Description
Rabbit polyclonal antibody to ARHGEF7

Applications 
WB, IHC.

Immunogen 
ARHGEF7 Antibody detects endogenous levels of total ARHGEF7.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(91%), Bovine(%), Horse(%), Sheep(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
75-80 KD; 90kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ARHGEF7 

Synonyms 
ARHG7; ARHG7_HUMAN; ARHGEF 7; Arhgef7; Beta pix; Beta-Pix; betaPix; betaPix-b; betaPix-c; Cool; COOL-1; COOL1; DKFZp686C12170; DKFZp761K1021; KIAA0142; KIAA0412; mKIAA0142; Nbla10314; P50; P50BP; p85; P85COOL1; P85SPR; PAK interacting exchange factor beta; PAK-interacting exchange factor beta; PAK3; Pak3bp; PIX; PIXB; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor (GEF) 7b; Rho guanine nucleotide exchange factor (GEF7); Rho guanine nucleotide exchange factor 7; SH3 domain containing proline rich protein;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q14155

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using ARHGEF7 Antibody at 1/1000 dilution. Observed bands:70kD....

Western blot analysis of extracts from Mouse testis, using ARHGEF7 Antibody at 1/1000 dilution. Observed bands:60kD....

Western blot analysis of extracts from EC304 cells(heat-shock treatment), using ARHGEF7 Antibody at 1/1000 dilution. Observed bands:60kD....

ab15293 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....