Guanine nucleotide exchange factor which activates Rho GTPases. Strongly activates RHOA. Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization. Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia. Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).

Description
Rabbit polyclonal antibody to ARHGEF5

Applications 
WB, IF, ICC, IHC.

Immunogen 
ARHGEF5 Antibody detects endogenous levels of total ARHGEF5.

Reactivity 
Human, Mouse, Rat.
可预测：Rabbit(89%), Dog(%)

Molecular weight
60 KD; 177kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ARHGEF5

Synonyms 
ARHG5_HUMAN; ARHGEF 5; ARHGEF5; DKFZp686N1969; ephexin 3; Ephexin-3; GEF 5; GEF5; Guanine nucleotide regulatory protein TIM; Oncogene TIM; P60; p60 TIM; Rho guanine nucleotide exchange factor (GEF) 5; Rho guanine nucleotide exchange factor 5; TIM 1; TIM; TIM1; Transforming immortalized mammary oncogene;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q12774

Western blot analysis of extracts from NIH-3T3 cells, using ARHGEF5 Antibody. The lane on the left was treated with blocking peptide....

ab15292 staining 3T3 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab15292 1:200) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....