Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Regulates reorganization of the actin cytoskeleton mediated by Rho family members.

Description
Rabbit polyclonal antibody to ARHGDIB

Applications 
WB, IF, ICC, IHC.

Immunogen 
ARHGDIB Antibody detects endogenous levels of total ARHGDIB.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
27 kDa; 23kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ARHGDIB

Synonyms 
Arhgdib; D4; D4 GDP dissociation inhibitor; GDIA 2; GDIA2; GDID 4; GDID4; GDIR2_HUMAN; GDP dissociation inhibitor D4; LY GDI; Ly-GDI; LYGDI; MGC108926; RAP1GN1; Rho GDI 2; Rho GDI beta; Rho GDI2; Rho GDP dissociation inhibitor (GDI) beta; Rho GDP dissociation inhibitor 2; Rho GDP dissociation inhibitor beta; Rho GDP-dissociation inhibitor 2; Rho-GDI beta; RhoGDI2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P52566

Western blot analysis of extracts from Mouse lung, using ARHGDIB Antibody. The lane on the left was treated with blocking peptide....

ab12970 at 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab12970 at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab12970 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab12970 at 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab12970 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab12970 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab12970 1:200) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....