GTPase-activating protein for Rho family members.

Description
Rabbit polyclonal antibody to ARHGAP5

Applications 
WB, IF, ICC, IHC.

Immunogen 
ARHGAP5 Antibody detects endogenous levels of total ARHGAP5.

Reactivity 
Human.
可预测：Pig(100%), Zebrafish(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)

Molecular weight
172 kDa; 172kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ARHGAP5

Synonyms 
ARHGAP 5; ARHGAP5; p190 B; p190 RhoGAP; Rho GTPase activating protein 5; RhoGAP 5; RhoGAP; RhoGAP5;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q13017

Western blot analysis of ARHGAP5 using HeLa whole lysates....

ab17561 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab17561 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab17561 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab17561 1:200) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....