Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.

Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival (By similarity). In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Description
Rabbit polyclonal antibody to ARG1

Applications 
WB, IF, ICC.

Immunogen 
ARG1 Antibody detects endogenous levels of total ARG1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(89%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)

Molecular weight
35 KD; 35kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ARG1

Synonyms 
A I; Al; ARG 1; arg1; ARGI1_HUMAN; Arginase 1; Arginase liver; Arginase type I; Arginase, liver; Arginase-1; Arginase1; Liver type arginase; Liver-type arginase; Type I arginase;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P05089
 

Western blot analysis of extracts from mouse brain , using ARG1 antibody....

ab14650 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14650 1:200) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....

Fig. 3. Metformin promoted microglia cells polarization from M1 phenotype to the M2 phenotype in the hippocampus of adult mice after lengthy isoflurane exposure. Representative western blots (above panels) and densitometric analysis (below panels, n = 3 mice per group) showing the effects of lengthy isoflurane inhalation (A, D), metformin pretreatment plus isoflurane inhalation (B, E), and metformin treatment (C, F) on expression levels of M1 microglia markers (CD68, iNOS) and M2 microglia markers (IL-10, Arg-1) in the hippocampus of adult mice. Tubulin was used as an internal control. Data are expressed as mean ± SD and compared by two-tailed Student’s t test....