GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Description
Rabbit polyclonal antibody to ARFGAP3

Applications 
WB, IF, ICC, IHC.

Immunogen 
ARFGAP3 Antibody detects endogenous levels of total ARFGAP3.

Reactivity 
Human, Mouse.
可预测：Pig(90%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
57kd,66kDa; 57kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ARFGAP3

Synonyms 
ADP ribosylation factor GTPase activating protein 1; ADP ribosylation factor GTPase activating protein 3; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ARFG3_HUMAN; ArfGAP 3; ARFGAP1; ARFGAP3; FLJ45618;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9NP61

Western blot analysis of extracts from HeLa cells, using ARFGAP3 antibody....

Western blot analysis of extracts from various samples, using ARFGAP3 Antibody. Lane 1: HepG2 cells(H2O2 treatment), blocked with antigen-specific peptides, Lane 2: HepG2 cells(H2O2 treatment), Lane 3: Mouse heart, Lane 4: Hela cells, Lane 5: Mouse kidney. Observed bands: 66kDa...

Western blot analysis of extracts from HeLa cells, using ARFGAP3 Antibody. The lane on the left was treated with blocking peptide....

ab14607 at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14607 at 1/100 staining mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14607 at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....