GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).

Description
Rabbit polyclonal antibody to ARFGAP1

Applications 
WB, IF, ICC.

Immunogen 
ARFGAP1 Antibody detects endogenous levels of total ARFGAP1.

Reactivity 
Human, Mouse, Rat.
可预测：Rabbit(88%)

Molecular weight
60/48 KD; 45kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ARFGAP1

Synonyms 
ADP ribosylation factor 1 GTPase activating protein; ADP ribosylation factor GTPase activating protein 1; ADP-ribosylation factor 1 GTPase-activating protein; ADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ARF1 directed GTPase activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein; ARF1GAP; ARFG1_HUMAN; Arfgap1; GAP protein; HRIHFB2281;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q8N6T3

Western blot analysis of extracts from various samples, using ARFGAP1 Antibody. Lane 1: Rat liver, blocked with antigen-specific peptides. Lane 2: Rat liver. Lane 3: Mouse brain在45左右. Lane 4: Hepg2 cells(heat-shock treatment)....

ab14606 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14606) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....