Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation. Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

Description
Rabbit polyclonal antibody to AR

Applications 
WB, IF, ICC, IHC.

Immunogen 
AR Antibody detects endogenous levels of total Androgen Receptor.

Reactivity 
Human, Mouse.
可预测：Pig(91%), Bovine(%), Sheep(%), Rabbit(%), Dog(%)

Molecular weight
110kDa; 99kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AR

Synonyms 
AIS; ANDR_HUMAN; Androgen nuclear receptor variant 2; Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease); Androgen receptor; androgen receptor splice variant 4b; AR; AR8; DHTR; Dihydro testosterone receptor; Dihydrotestosterone receptor (DHTR); Dihydrotestosterone receptor; HUMARA; HYSP1; KD; Kennedy disease (KD); NR3C4; Nuclear receptor subfamily 3 group C member 4 (NR3C4); Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; Spinal and bulbar muscular atrophy (SBMA); Spinal and bulbar muscular atrophy; Testicular Feminization (TFM); TFM;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P10275
 

Western blot analysis of extracts from Mouse brain, using Androgen Receptor Antibody. Lane 1 was treated with the blocking peptide....

ab11368 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#ab11368) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue)....

Supplementary Figure 2. |Immunohistochemical examination of 17βHSD3 and AR expression in tumor tissue. (A) Immunohistochemical labeling of 17βHSD3-positive cells. Arrows indicate 17βHSD3 expression.(C) Quantitative analysis of 17βHSD3-expressing cells using ImageJ software. (B) Immunohistochemical labeling of AR-positive cells. Arrows indicate AR expression....

Fig. 5 Effect of CEFFE (0, 50, 250, 500 μg/ml; 72 h), DHT (1, 10, 100 μM; 72 h) and DHT + CEFFE (100 μM DHT + 50, 250, 500 μg/ml CEFFE; 72 h) on the intracellular DHT concentration and AR expression of hDPCs. A Quantitative analysis of DHT concentration using ELISA. B RT-qPCR analysis of AR mRNA expression after culturing hDPCs with CEFFE, DHT and DHT + CEFFE, respectively. qRT-PCR analysis showed the expression of AR was increased after DHT treatment but declined after co-incubation with DHT and CEFFE. C Western blot for AR after treating hDPCs with CEFFE, DHT and DHT + CEFFE. Original data was presented in Additional file 2: Fig. 4. It revealed an increase in the expression of AR after incubating with DHT but decreased after co-incubation with DHT and CEFFE. Data represent the mean ± SD; n = 3; *P ...