Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core (By similarity). Plays a role in cell-to-cell adhesion and facilitates gap junction coupling.

Description
Rabbit polyclonal antibody to AQP0

Applications 
WB, IF, ICC, IHC.

Immunogen 
AQP0 Antibody detects endogenous levels of total AQP0.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(91%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)

Molecular weight
37 kDa; 28kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AQP0 

Synonyms 
AQP 0; AQP0; Aquaporin; Aquaporin-0; Aquaporin0; Lens fiber major intrinsic protein; LIM 1; LIM1; Major intrinsic protein 26kD; Major intrinsic protein of lens fiber; MIP 26; mip; MIP_HUMAN; MIP26; MP 26; MP26; PALM;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P30301

Western blot analysis of extracts from mouse eye tissue, using AQP0 Antibody. The lane on the left was treated with blocking peptide....

Western blot analysis of AQP0 Antibody expression in mouse eye tissue lysates.The lane on the right was treated with the antigen-specific peptide....

ab10987 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab10987) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue)....

AF5194 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab10987) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue)....