Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (By similarity). Regulates TLR4 signaling in activated macrophages (By similarity). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex.

Description
Rabbit polyclonal antibody to APPL1

Applications 
WB, IF, ICC, IHC.

Immunogen 
APPL1 Antibody detects endogenous levels of total APPL1.

Reactivity 
Human, Mouse.
可预测：Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)

Molecular weight
80 KD; 80kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
APPL1

Synonyms 
Adapter protein containing PH domain; Adaptor Protein Containing pH Domain; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1; Adaptor protein containing pH domain PTB domain and leucine zipper motif; AKT2 INTERACTOR; APPL 1; Appl1; DCC interacting protein 13 alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13 alpha; Dip13-alpha; DIP13alpha; DP13A_HUMAN; KIAA1428; PTB domain and leucine zipper motif 1; PTB Domain and Leucine Zipper Motif; Signaling adaptor protein DIP13alpha;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9UKG1

Western blot analysis of extracts from 293, using APPL1 Antibody....

ab14842 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab14842 at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody....