Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ).

Description
Rabbit polyclonal antibody to APLF

Applications 
WB, IF, ICC, IHC.

Immunogen 
APLF Antibody detects endogenous levels of total APLF.

Reactivity 
Human, Mouse.
可预测：Pig(86%), Bovine(%), Horse(%), Sheep(%), Dog(%)

Molecular weight
57kDa; 57kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
APLF

Synonyms 
2010301N04Rik; AI452191; Aplf; APLF_HUMAN; Aprataxin and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; C2orf13; PNK and APTX like FHA protein; PNK and APTX-like FHA domain-containing protein; RGD1565557; XIP1; XRCC1 interacting protein 1; XRCC1-interacting protein 1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q8IW19

ab10028 staining A549 cells(H2O2 treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#ab10028) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue)....