Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

Description
Rabbit polyclonal antibody to API-5

Applications 
WB, IF, ICC, IHC.

Immunogen 
API-5 Antibody detects endogenous levels of total API-5.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(89%), Horse(%), Sheep(%), Dog(%)

Molecular weight
58 KD; 59kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
API-5 

Synonyms 
AAC 11; AAC-11; AAC11; Antiapoptosis clone 11 protein; API 5; API-5; api5; API5 like 1; API5_HUMAN; API5L1; Apoptosis inhibitor 5; Cell migration inducing gene 8 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; Fibroblast growth factor 2-interacting factor; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9BZZ5

Western blot analysis of extracts from various samples, using API-5 Antibody. Lane 1: Mouse brain, blocked with antigen-specific peptides. Lane 2: Mouse brain. Lane 3: Hepg2 cells(heat-shock treatment). Lane 4: Hela cells(heat-shock treatment)....

ab14375 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14375) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....