Endogenous ligand for the apelin receptor (APLNR). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity).

(Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives.

Description
Rabbit polyclonal antibody to Apelin

Applications 
WB, IF, ICC, IHC.

Immunogen 
Apelin Antibody detects endogenous levels of total Apelin.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(%), Sheep(%), Rabbit(%)

Molecular weight
8kDa; 9kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
Apelin

Synonyms 
AGTRL1 ligand; APEL; APEL_HUMAN; Apelin-13; APJ endogenous ligand; Apln; XNPEP2;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9ULZ1
 

Western blot analysis of extracts from Hela cells, using Apelin Antibody. The lane on the left was treated with blocking peptide....

ab13159 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab13159 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab13159 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....

Figure 5. Immunohistochemical analysis of CD34, vascular endothelial growth factor (VEGF), and angiogenesis-related differentially expressed genes in the experimental (alveolar echinococcosis [AE]) and control (AE control group [AEC]) group samples. (A) CD34 and VEGF staining in AE samples showed different degrees of vascularization. Increased expression of SPP1, RSPO3, APLN, TWIST1, ADAM12, and FOXC2 was observed in the AE samples, whereas low or no expression of these genes was observed in the AEC samples. (B) The H-scores of CD34, VEGF, SPP1, RSPO3, APLN, TWIST1, ADAM12, and FOXC2 were significantly higher in the AE samples than in the AEC samples (values represent the mean ± standard error of the mean: ∗∗∗P < .001, ∗∗∗∗P < .0001)....

Figure 2 The expression of apelin in DR mice treated with LV-EGFP, LV-Apelin+, or LV-Apelin−. (A) Representative apelin staining images by CLSM and (B) the quantification of the staining intensity indicated that apelin was moderately expressed in the LV-EGFP group and was primarily distributed in the GCL, while it was widely expressed in the LV-Apelin+ group and distributed in the GCL and INL. In the LV-Apelin− group, there was almost no expression of apelin. (C) The qRT-PCR results revealed that the mRNA expression of apelin was upregulated in the LV-Apelin+ group, but downregulated in the LV-Apelin− group. Blue: DAPI, red: apelin. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer. Data were expressed as mean ± SD. LV-Apelin+ versus LV-EGFP; LV-Apelin− versus LV-EGFP;...