Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity).

Description
Rabbit polyclonal antibody to Annexin A1

Applications 
WB, IF, ICC, IHC.

Immunogen 
Annexin A1 Antibody detects endogenous levels of total Annexin A1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(81%), Bovine(%), Horse(%), Sheep(%), Rabbit(%)

Molecular weight
38 kDa; 39kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
Annexin A1 

Synonyms 
Annexin 1; Annexin A1; Annexin I (lipocortin I); Annexin I; Annexin-1; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; ANXA1_HUMAN; Calpactin 2; Calpactin II; Calpactin-2; CalpactinII; Chromobindin 9; Chromobindin-9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; LPC 1; LPC1; p35; Phospholipase A2 inhibitory protein;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P04083

Western blot analysis of Annexin I expression in Mouse brain lysate...

Western blot analysis of Annexin I Antibody expression in mouse liver tissue lysates.The lane on the right was treated with the antigen-specific peptide....

ab10947 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab10947 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

Fig. 3. Allopurinol upregulates junctional protein expression by suppressing TLR4/P65. (A–C) Representative immunoblotting of TLR4 (A), P65 (B) and IL-1β (C) from isolated colon after treatment with allopurinol or antibiotic. (D–F) Relative expression levels of Occludin (D), ZO-1 (E) and Claudin1 (F) genes were determined by Q-PCR from isolated colon after treatment with allopurinol or antibiotic. n = 6 mice per group. (G and H) Representative immunoblotting of Occludin, Claudin1（G）and IL-1β（H）from isolated colon after treatment with allopurinol or antibiotic. NC, control group; HY, hyperuricemia mice. Bars and error bars represent the Means ± SEM. *p < 0.05, **p < 0.01 verse Con, ##p < 0.01 verse HY using one-way ANOVA followed by Tukey...

Figure 4. Intestinal barrier permeability and intestinal-barrier-related protein expression. N = 8. (A) Serum diamine oxidase activity and (B) D-lactate contents. (C–F) The relative protein expression of ZO-1, Occludin, and MUC2 in the jejunum. CON, basal diet; EQ, basal diet + 200 g/ton ethoxyquin; AC, basal diet + 200 g/ton antioxidant compound. Abbreviations: DAO, diamine oxidase. ab Different superscript letters indicate significant differences (p < 0.05)....