Description
Rabbit polyclonal antibody to AN30A

Applications 
WB, IF, ICC, IHC.

Immunogen 
AN30A Antibody detects endogenous levels of total AN30A.

Reactivity 
Human.

Molecular weight
153 KD; 159kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AN30A

Synonyms 
AN30A_HUMAN; ANKRD30A; ankyrin repeat domain 30A; Ankyrin repeat domain containing protein 30A; Ankyrin repeat domain-containing protein 30A; breast cancer antigen NY-BR-1; NY BR 1; RP11 20F24.1; Serologically defined breast cancer antigen NY BR 1; Serologically defined breast cancer antigen NY-BR-1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9BXX3

ab14285 at 1/100 staining human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14285 at 1/100 staining human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14285 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14285 at 1/100 staining human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14285 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14285) and mouse anti-beta tubulin for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....