Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity).

Description
Rabbit polyclonal antibody to ALKBH5

Applications 
WB, IHC.

Immunogen 
ALKBH5 Antibody detects endogenous levels of total ALKBH5.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(92%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)

Molecular weight
44 kDa, 37 kDa; 44kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ALKBH5

Synonyms 
ABH5; AlkB, alkylation repair homolog 5 (E. coli); AlkB, alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha ketoglutarate dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; Oxoglutarate and iron-dependent oxygenase domain containing; Probable alpha ketoglutarate dependent dioxygenase ABH5; RNA demethylase ALKBH5;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q6P6C2

Western blot analysis of extracts from various samples, using ALKBH5 Antibody. Lane 1: Mouse heart, treated with blocking peptide; Lane 2: Mouse heart; Lane 3: Rat muscle. Observed bands: 37 kDa....

ab13531 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

FIGURE 7 Verification of m6A methylation level and methylase expression in LF. (A) The m6A methylation level in LF. (B) Semiquantitative analysis of m6A methylation. (C) IGV plots of the WTAP m6A level. (D) IGV plots of the ALKBH5 m6A level. (E) IGV plots of the YTHDF1 m6A level. (F) IGV plots of the WTAP expression level. (G) IGV plots of the ALKBH5 expression level. (H) IGV plots of the YTHDF1 expression level. (I) mRNA expression level of WTAP. (J) mRNA expression level of ALKBH5. (K) mRNA expression level of YTHDF1. (L) Protein expression levels of WTAP. (M) Protein expression levels of ALKBH5. (N) Protein expression levels of YTHDF1. (O) Semiquantitative analysis of WTAP protein. (P) Semiquantitative analysis of YTHDF1 protein. (Q) Semiquantitative analysis of ALKBH5 protein. ## p < 0.01 compared with the control group, # p < 0.05 compared with the control group....