Scaffold protein that plays an important role in assembling signaling complexes downstream of several types of G protein-coupled receptors. Activates RHOA in response to signaling via G protein-coupled receptors via its function as Rho guanine nucleotide exchange factor. May also activate other Rho family members. Part of a kinase signaling complex that links ADRA1A and ADRA1B adrenergic receptor signaling to the activation of downstream p38 MAP kinases, such as MAPK11 and MAPK14. Part of a signaling complex that links ADRA1B signaling to the activation of RHOA and IKBKB/IKKB, leading to increased NF-kappa-B transcriptional activity. Part of a RHOA-dependent signaling cascade that mediates responses to lysophosphatidic acid (LPA), a signaling molecule that activates G-protein coupled receptors and potentiates transcriptional activation of the glucocorticoid receptor NR3C1. Part of a signaling cascade that stimulates MEF2C-dependent gene expression in response to lysophosphatidic acid (LPA) (By similarity). Part of a signaling pathway that activates MAPK11 and/or MAPK14 and leads to increased transcription activation of the estrogen receptors ESR1 and ESR2. Part of a signaling cascade that links cAMP and EGFR signaling to BRAF signaling and to PKA-mediated phosphorylation of KSR1, leading to the activation of downstream MAP kinases, such as MAPK1 or MAPK3. Functions as scaffold protein that anchors cAMP-dependent protein kinase (PKA) and PRKD1. This promotes activation of PRKD1, leading to increased phosphorylation of HDAC5 and ultimately cardiomyocyte hypertrophy (By similarity). Has no guanine nucleotide exchange activity on CDC42, Ras or Rac. Required for normal embryonic heart development, and in particular for normal sarcomere formation in the developing cardiomyocytes (By similarity). Plays a role in cardiomyocyte growth and cardiac hypertrophy in response to activation of the beta-adrenergic receptor by phenylephrine or isoproterenol. Required for normal adaptive cardiac hypertrophy in response to pressure overload. Plays a role in osteogenesis (By similarity).

Description
Rabbit polyclonal antibody to AKAP13

Applications 
WB, IF, ICC, IHC.

Immunogen 
AKAP13 Antibody detects endogenous levels of total AKAP13.

Reactivity 
Human, Mouse.

Molecular weight
307 KD; 308kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AKAP13

Synonyms 
A kinase (PRKA) anchor protein 13; A kinase anchor protein 13; A kinase anchoring protein; A-kinase anchor protein 13; AKAP 13; AKAP Lbc; AKAP-13; AKAP-Lbc; AKAP13; AKP13_HUMAN; ARHGEF13; Breast cancer nuclear receptor binding auxiliary protein; Breast cancer nuclear receptor-binding auxiliary protein; BRX; c lbc; FLJ11952; FLJ43341; Guanine nucleotide exchange factor Lbc; HA 3; HA3; Ht 31; Ht31; Human thyroid anchoring protein 31; Human thyroid-anchoring protein 31; LBC; LBC oncogene; Lymphoid blast crisis oncogene; Non oncogenic Rho GTPase specific GTP exchange factor; Non-oncogenic Rho GTPase-specific GTP exchange factor; p47; Protein kinase A anchoring protein 13; Protein kinase A-anchoring protein 13; PROTO LB; PROTO LBC;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q12802

Western blot analysis of extracts from Jurkat cells using AKAP13 antibody.The lane on the left was treated with the antigen-specific peptide....

ab14114 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14114 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....