Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.

Description
Rabbit polyclonal antibody to AIM2

Applications 
WB, IF, ICC.

Immunogen 
AIM2 Antibody detects endogenous levels of total AIM2.

Reactivity 
Human, Mouse.
可预测：Horse(88%)

Molecular weight
39 KD; 39kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AIM2

Synonyms 
Absent in melanoma 2; AIM 2; Aim2; AIM2_HUMAN; Interferon-inducible protein AIM2; OTTHUMP00000035296; PYHIN4;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
O14862

Western blot analysis of extracts from mouse brain, using AIM2 Antibody. Lane 1 was treated with the antigen-specific peptide....

Western blot analysis of extracts from Mouse brain, using AIM2 Antibody. The lane on the left was treated with blocking peptide....

Figure 4 Effect of AICAR treatment on NLRP3, NLRP1, NLRC4, and AIM2 inflammasome components (A,C) Representative immunofluorescence staining images showing the expressions of NLRP3 and caspase-1 in the spinal dorsal horn of sham and CIBP rats. Scale bar: 20 μm. (B,D) Quantitative analysis of fluorescence intensity in (A) and (C). Data are expressed as the mean±SD ( n=3). * P...

Fig. 5. Effects of Rg1 treatment on inflammation in LPS-induced CKD mice. (A) Representative WB images of AIM2, Caspase-1 p20, IL-6, and Cleaved-IL-1β. (B) Statistical results of AIM2. (C) Statistical results of Caspase-1 p20. (D) Statistical results of IL-6. (E) Statistical results of Cleaved-IL-1β. (F) Statistical results of Il1b and Casp1 mRNA (qPCR). (G) Representative images of AIM2 (IF, ×400, bar: 20 μm). (H) Statistical results of AIM2 IF. Data are expressed as mean ± SD, WB and IF, n = 4, qPCR, n = 3. **P < 0.01 versus Control; #P < 0.05, ##P < 0.01 versus Model....

Fig. 5. Effects of Rg1 treatment on inflammation in LPS-induced CKD mice. (A) Representative WB images of AIM2, Caspase-1 p20, IL-6, and Cleaved-IL-1β. (B) Statistical results of AIM2. (C) Statistical results of Caspase-1 p20. (D) Statistical results of IL-6. (E) Statistical results of Cleaved-IL-1β. (F) Statistical results of Il1b and Casp1 mRNA (qPCR). (G) Representative images of AIM2 (IF, ×400, bar: 20 μm). (H) Statistical results of AIM2 IF. Data are expressed as mean ± SD, WB and IF, n = 4, qPCR, n = 3. **P < 0.01 versus Control; #P < 0.05, ##P < 0.01 versus Model....