Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation.

Description
Rabbit polyclonal antibody to AF10

Applications 
WB, IF, ICC, IHC.

Immunogen 
AF10 Antibody detects endogenous levels of total AF10.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)

Molecular weight
109 KD; 113kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AF10

Synonyms 
AF 10; AF10; AF10_HUMAN; ALL 1 fused gene from chromosome 10; ALL1 fused gene from chromosome 10; ALL1-fused gene from chromosome 10 protein; DKFZp686E10210; MGC75086; MLLT 10; MLLT10; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10; Myeloid/lymphoid or mixed lineage leukemia translocated to 10; OTTHUMP00000045273; Protein AF 10; Protein AF-10; Protein AF10; Type I AF10 protein;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P55197

ab14081 at 1/100 staining human Testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody....