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Western blot analysis of extracts from HeLa cells, using ADRB2 antibody.The lane on the left was treated with the antigen-specific peptide....
ab14372 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
Figure 6. 16α-OHE1 alleviates cardiomyocyte damage. (A-B) ELISA kits were used to measure creatine kinase isoenzyme (CK-MB) and troponin T (cTnT) levels in H9C2 cells. (C, D) Relative protein levels of brain natriuretic peptide (BNP) in H9C2 cells. (E, F) Relative protein levels of β2-adrenoceptor (β2AR) in H9C2 cells. (G-I) ELISA kits were used to measure interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) levels in H9C2 cells. (J) The viability of H9C2 cells exposed to hypoxia for 24 was assessed by CCK-8 assays. (K) Apoptosis in H9C2 cells incubated under hypoxic conditions for 24 h was evaluated by flow cytometry. n = 3. *p < 0.05, **p < 0.01 vs. N; #p < 0.05, ##p < 0.01 vs. H. The data were presented as the mean ± SEM. (one-way ANOVA)...

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