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ab11349 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody....
Fig. 6| Targeting of VEGFA represses EHD1-induced tumor growth and angiogenesis in vivo. a-b The expression of EHD1, β2AR and VEGFA in freshly frozen xenograft tumor tissue was analyzed by Western blot....
Figure S9. |Correlation of EHD1 with β2AR, VEGFA and CD31 expression and angiogenesis in HCC tissues. a Representative IHC images of NSCLC samples with low and high expression of the indicated proteins. Scale bar, 50 μm and 100 μm....

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