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Figure 6. |Density A1R and A2AR analysed by Western blot in nerve terminal-enriched membranes(A) and total membranes (B) from the hippocampus of GK and Wistar (W) rats, either receiving 1g/L caffeine (Caff) or tap water. Samples were loaded in SDS-PAGE gels at a protein concentration of 40 or 150 μg for A1R (n=6-8) and A2AR (n=2-3), respectively. Immunoreactivity was normalised to either α-tubulin or β-actin, and calculated as percentage of control in the same Western blot experiment. Symbols represent LSD test results after ANOVA with either significant diabetes effect or significant diabetes-caffeine interaction: *P<0.05, **P<0.01 for GK versus Wistar in the respective treatment group or as indicated....

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