Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2 and GRIK2) and serotonin (HTR2C), GABA receptor (GABRA3) and potassium voltage-gated channel (KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1 (HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.

Description
Rabbit polyclonal antibody to ADARB1

Applications 
WB, IF, ICC, IHC.

Immunogen 
ADARB1 Antibody detects endogenous levels of total ADARB1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Molecular weight
80 KD; 81kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ADARB1

Synonyms 
ADARB 1; ADARB1; 1700057H01Rik; ADAR2; ADAR2a; ADAR2a L1; ADAR2a L2; ADAR2a L3; ADAR2b; ADAR2c; ADAR2d; ADAR2g; Adarb1; Adenosine deaminase, RNA specific, 2; Adenosine deaminase, RNA specific, B1 (homolog of rat RED1); Adenosine deaminase, RNA specific, B1 (RED1 homolog rat); Adenosine deaminase, RNA specific, B1; AW124433; AW558573; BB220382; D10Bwg0447e; Double stranded RNA specific editase 1; Double-stranded RNA-specific editase 1; DRABA2; DRADA2; dsRNA adenosine deaminase; EC 3.5.-.-; Human dsRNA adenosine deaminase DRADA2; Human dsRNA adenosine deaminase DRADA2b, EC 3.5; OTTHUMP00000115341; OTTHUMP00000115342; RED 1; RED1_HUMAN; RNA editase; RNA editase 1; RNA editing deaminase 1; RNA editing enzyme 1; RNA editing enzyme 1, rat, homolog of; RNA specific adenosine deaminase B1; RNA-editing deaminase 1; RNA-editing enzyme 1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P78563

Western blot analysis of extracts from 293, using ADARB1 Antibody. Lane 1 was treated with the blocking peptide....

ab14092 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab14092 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab14092 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab14092 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....