Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity).

Description
Rabbit polyclonal antibody to ADAM 17

Applications 
WB, IF, ICC.

Immunogen 
ADAM 17 Antibody detects endogenous levels of total ADAM 17.

Reactivity 
Human, Mouse, Rat.
可预测：Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Molecular weight
93 kDa; 93kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ADAM 17

Synonyms 
A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme); A disintegrin and metalloproteinase domain 17; ADA17_HUMAN; ADAM 17; ADAM metallopeptidase domain 17; ADAM17; ADAM17 protein; CD 156b; CD156b; CD156b antigen; CSVP; Disintegrin and metalloproteinase domain-containing protein 17; MGC71942; NISBD; NISBD1; Snake venom like protease; Snake venom-like protease; TACE; TNF alpha convertase; TNF alpha converting enzyme; TNF-alpha convertase; TNF-alpha-converting enzyme; Tumor Necrosis Factor Alpha Converting Enzyme;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P78536

Western blot analysis of ADAM 17 expression in HT1080 whole cell lysates....

ab11550 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab11550) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....

ab11550 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab11550 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....

Fig. 6. |ADAM17 suppression enhanced matrix metabolism in NP cells.(A,B) Western blotting (A) and subsequent densitometric analysis (B) revealed that collagen IIa and aggrecan protein expression in rat NP cells was increased by the ADAM17 inhibitor. GAPDH was used as the control. (C) Immunofluorescence imaging of human NP cells treated with LV-shControl and LV-shADAM17. (D, E)Western blotting (D) and subsequent densitometric analysis (E) demonstrated that ADAM17 suppression by LV-shADAM17 markedly increased collagen IIa and aggrecan protein expression in human NP cells....

Fig. 6. Change in macrophage secreted ADAM-17 in vitro and in vivo. Levels of ADAM-17 in BALF (A, n = 5 per group) and expression levels of ADAM-17 in mouse lung tissues (B, n = 3 per group). C Levels of ADAM-17 in cell supernatants from differently polarized macrophages. D Representative image and densitometry of immunofluorescence of ADAM-17 in different polarized macrophages (Scar bar, 20 μm). Relative protein expression E and mRNA F of ADAM-17 in different polarized macrophages. Based on three independent experiments, all data were shown as mean ± SD....

Fig. 6. Change in macrophage secreted ADAM-17 in vitro and in vivo. Levels of ADAM-17 in BALF (A, n = 5 per group) and expression levels of ADAM-17 in mouse lung tissues (B, n = 3 per group). C Levels of ADAM-17 in cell supernatants from differently polarized macrophages. D Representative image and densitometry of immunofluorescence of ADAM-17 in different polarized macrophages (Scar bar, 20 μm). Relative protein expression E and mRNA F of ADAM-17 in different polarized macrophages. Based on three independent experiments, all data were shown as mean ± SD....