Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well.

Description
Rabbit polyclonal antibody to ACVL1

Applications 
WB, IF, ICC, IHC

Immunogen 
ACVL1 Antibody detects endogenous levels of total ACVL1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Molecular weight
56 KD; 56kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ACVL1

Synonyms 
Activin A receptor; Activin A receptor type II like 1; Activin A receptor, type II like kinase 1; Activin receptor like kinase 1; Activin receptor-like kinase 1; ACVL1_HUMAN; ACVRL1; ACVRLK1; ALK-1; ALK1; HHT; HHT2; ORW2; Osler Rendu Weber syndrome 2; Serine/threonine protein kinase receptor R3; Serine/threonine-protein kinase receptor R3; SKR3; TGF B superfamily receptor type I; TGF-B superfamily receptor type I; TSR-I; TSR1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P37023

ab14035 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14035) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue)....

Figure 3 BMP9 activates ALK1/Smad1/5 signaling in HFL-1 cells. Effect of (A) different concentrations of BMP9 and (B) incubation with BMP9 for different time periods on the protein expression levels of ALK1, p-Smad1/5 and t-Smad1/5. BMP9, bone morphogenetic protein 9; ALK1, activin receptor-like kinase 1; p, phosphorylated; t, total; ctrl, control....

Figure 4. |ALK1 inhibitor Dor suppresses the effects of BMP9 on HFL-1 cells.(A) Protein expression levels of α-SMA, collagen I and collagen III....