F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Description
Rabbit polyclonal antibody to Actinin alpha 2/3

Applications 
WB, IF, ICC, IHC

Immunogen 
Actinin alpha 2/3 Antibody detects endogenous levels of total Actinin alpha 2/3.

Reactivity 
Human, Mouse, Rat.
可预测：Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)

Molecular weight
103 KD; 104kD,103kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
Actinin  alpha 2/3

Synonyms 
Actin binding protein; Actinin alpha 2; ACTN 2; ACTN2; ACTN2_HUMAN; Alpha actinin 2; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 2; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; CMD1AA; F actin cross linking protein; F-actin cross-linking protein; Actinin alpha 3; actinin, alpha; ACTN 3; ACTN3; ACTN3_HUMAN; Alpha actinin skeletal muscle; Alpha actinin skeletal muscle isoform 3; Alpha-Actinin 3; Alpha-actinin skeletal muscle isoform 3; Alpha-actinin-3; F actin cross linking protein; F-actin cross-linking protein; MGC117002; MGC117005; OTTHUMP00000219049; OTTHUMP00000219054;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P35609 | Q08043

Western blot analysis of extracts from HepG2 cells(H2O2 treatment), using Actinin alpha 2/3 Antibody. The lane on the left was treated with blocking peptide....

Western blot analysis of extracts from various samples, using Actinin alpha 2/3 Antibody. Lane 1: Mouse brain lysates; Lane 2: Rat brain lysates; Lane 3: Mouse muscle lysates; Lane 4: Rat spleen lysates;...

Western blot analysis of extracts from various samples, using Actinin alpha 2/3 Antibody. Lane 1: Mouse brain, blocked with antigen-specific peptides, Lane 2: Mouse brain, Lane 3: Hela cells(serum starvation treatment), Lane 4: RAW264.7 cells(LPS 4h treatment)....

ab14029 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab14029 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab14029 at 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab14029 at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....