Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

Description
Mouse monoclonal antibody to actin

Applications 
WB, IF, ICC, IHC

Immunogen 
Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.

Reactivity 
Human,Mouse,Rat,Pig,Zebrafish,Bovine,Sheep,Rabbit,Goat,Guinea pig,Dog,Monkey,Hamster,Chicken,Fish.

Molecular weight
43KD; 42kD(Calculated).

Host species 
Mouse

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
actin

Synonyms 
ACTB;Actin; cytoplasmic 1; Beta-actin; Beta actin; BRWS1; Actin; beta; Beta cytoskeletal actin; PS1TP5-binding protein 1; PS1TP5BP1;

Storage
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P60709

Western blot analysis of various lysates, using actin Mouse Monoclonal Antibody. Lane1:C6 cells treated with blocking peptide, Lane2:C6 cell lysates, Lane3:Hela cell lysates, Lane4:K562 cell lysates, Lane5:Mouse brain tissue lysates....