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ab11124 Actin alpha 1 Antibody
货号:
实验方法:WB,IP,IF,ICC
实验种属:Human,Mouse,Rat,Rabbit,Bovine,Chicken,Dog,Sheep,Horse
宿主种属:Rabbit
共轭标记:Unconjugated
产品售价:
产品订购:
jiamay@gmail.com
渠道电话:
+86 400-698-4168
客服电话:
+86 400-698-4168
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产品详情
产品应用信息
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Description
Rabbit polyclonal antibody to Actin alpha 1

Applications 
WB, IF, ICC, IHC, IP.

Immunogen 
Actin alpha 1 Antibody detects endogenous levels of total Actin alpha 1.

Reactivity 
Human, Mouse, Rat.
可预测:Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Molecular weight
42 kDa; 42kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
Actin alpha 1

Synonyms 

a actin; ACTA; ACTA1; ACTA2; ACTC; ACTC1; Actin; ACTS_HUMAN; ACTSA; Alpha 2 actin; alpha skeletal muscle; Alpha-actin-1; Cardiac muscle alpha actin 1; Skeletal muscle alpha actin 1;


Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P68133

图片数据
Western blot analysis of extracts from Mouse heart, using Actin alpha 1 Antibody. The lane on the left was treated with blocking peptide....
Western blot analysis of extracts from various tissue sample, using Actin α 1 Antibody. Lane1: mouse heart tissue, Lane2: mouse heart tissue, Lane3: rat heart tissue, Lane4: rat muscle...
Western blot analysis of extracts from various samples, using Actin alpha 1 Antibody. Lane 1: Rat heart, blocked with antigen-specific peptides, Lane 2: Rat heart, Lane 3: Mouse muscle, Lane 4: Rat brain, Lane 5: Mouse heart....
ab11124 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 at 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab11124 staining HepG2 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab11124 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....
Fig. 6 Protein expression levels in colon tissue samples examined by immunoblot. A TLR4, NF-κB, p-NF-κB and IL-1β. B PI3K, p-PI3K, AKT and p-AKT. Staining intensity was assessed with ImageJ. Data are mean ± SD, #P < 0.05, ##P < 0.01 versus normal group; *P < 0.05, **P < 0.01 versus Model group...

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