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Western blot analysis of extracts from mouse brain, using ACOX1 antibody. Lane 1 was treated with the blocking peptide....
ab12667 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
ab12667 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....
Fig 3. |Ambient PM exposure leads to hepatic steatosis by impairing hepatic lipid metabolism. (A) Oil Red O staining observation of liver (×200, scale bars = 100 μm). (B) H&E staining observation of liver (×200, scale bars = 50 μm). (C) The volume density of quantitation of hepatic steatosis (n = 5). (D) The genes expression involved in fatty acid β-oxidation in liver (n = 5). (E) The mRNA expression of genes involved in lipogenesis and FXR in liver (n = 5). (F) Bands of PPARα,PPARγ, ACOX1, FAS, SREBP-1c, SCD1....
Fig. 6. β-PAE promotes the expression of hepatic lipid oxidation-related proteins and genes in HFD-fed rats. (A–G) Western blot analysis on the expression of SIRT1, PGC-1α, PPARα, FGF21, CPT-1a and ACOX1; (H–K) The mRNA expression of SIRT1, PPARα, CPT-1a and ACOX1. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group....
FIGURE 6 Effect of CANA on the cardiac protein expression. (A) Heat map of individual genes within selected pathways, colored by the log2fold change; (B) Selected genes (Acadsb, Ndufb4, Pdk4, Acox1, Bdh1, and Ehhadh) were validated by Western blotting; (C,D) Quantitative evaluation of the protein expression of selected genes (Acadsb, Ndufb4, Pdk4, Acox1, Bdh1, and Ehhadh). * p < 0.05, ** p < 0.01 vs. NSD. # p < 0.05 vs. HSD....
Fig. 3 Antagomir-103-3p alleviated the damage to mice with NAFLD. Mice with NAFLD were fed an HFD for 8 weeks, and Antagomir-NC or Antagomir-103-3p was used for tail vein injection once a week for 2 weeks. A MiR-103-3p expression in mouse liver tissues was examined by qRT-PCR. B Oil Red O staining detected lipid droplet accumulation in mouse liver tissues, and HE staining detected liver tissue lesions in mice. C The TG, ALT, AST and H2O2 contents in mouse serum were examined, while ROS generation and ATP content were examined in mouse tissues. D The protein and mRNA levels of ACOX1, FASN and ACSL1 were examined by western blotting and qRT-PCR, respectively. *P < 0.05 compared with the control group; #P < 0.05 compared with the NAFLD+Antagomir-NC group...
Figure 2 Amelioration of lipid accumulation in PA-induced HepG2 cells by FV. (a) Oil red O was used to measure the level of lipid accumulation (magnification 100x, scale bar = 250 μm). (b) The oil red O-positive area was analyzed and quantified. (c–j) The relative mRNA expression levels of FABP1, SCD1, CD36, HMGCR, ACACA, CCL5, IL-1β, and TNF-α were determined by qRT-PCR. (k) The proinflammatory factor protein levels of IL-1β, IL-6, and TNF-α. (l) The protein levels related to lipid metabolism of SREBP-1, ACOX1, CD36, CPT1α, and HMGCR were analyzed by western blotting, and the relative ratios were calculated and expressed as the mean ± SD; n = 3. #P < 0.05 means that the difference between the NC group and the PA group is significant. ∗P < 0.05 means that the difference between the PA group and the FV (30 mg/mL) group or the FV (15 mg/mL) group is significant....
Fig. 2 Fenofibrate treatment attenuated fibrosis in a rat model of renal transplantation. A Schematic view of the treatment of fenofibrate in a rat model of renal transplantation. B Gross morphology of bilateral kidneys from rat underwent kidney transplantation (KT). C IF detected the expression of ACOX1 and α-SMA in transplanted kidney of rats, cell nuclei were strained in blue, renal tubular epithelial cells were stained green, ACOX1 was stained violet, and α-SMA was stained red. Scale bar: 50 μm. D HE, Masson, and Sirius Red staining in transplanted kidney from rat models with or without fenofibrate treatment. Scale bar: 50 μm. E Relative fibrosis level compared fenofibrate treatment group with control group. Significance was determined by Student’s t test....
Fig. 2 Fenofibrate treatment attenuated fibrosis in a rat model of renal transplantation. A Schematic view of the treatment of fenofibrate in a rat model of renal transplantation. B Gross morphology of bilateral kidneys from rat underwent kidney transplantation (KT). C IF detected the expression of ACOX1 and α-SMA in transplanted kidney of rats, cell nuclei were strained in blue, renal tubular epithelial cells were stained green, ACOX1 was stained violet, and α-SMA was stained red. Scale bar: 50 μm. D HE, Masson, and Sirius Red staining in transplanted kidney from rat models with or without fenofibrate treatment. Scale bar: 50 μm. E Relative fibrosis level compared fenofibrate treatment group with control group. Significance was determined by Student’s t test....
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