Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating intracellular levels of acyl-CoAs, free fatty acids and CoASH. More active towards saturated and unsaturated long chain fatty acyl-CoAs (C12-C20).

Description
Rabbit polyclonal antibody to ACOT1

Applications 
WB, IF, ICC, IHC

Immunogen 
ACOT1 Antibody detects endogenous levels of total ACOT1.

Reactivity 
Human.

Molecular weight
46 KD; 46kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ACOT1

Synonyms 
ACH2; Acyl CoA thioesterase 1, cytosolic; Acyl coenzyme A thioesterase 1; CTE 1; CTE I; CTE Ib; Cte1; Cytosolic acyl CoA thioesterase 1; D12Ucla1; Inducible cytosolic acyl-coenzyme A thioester hydrolase; Long chain acyl CoA hydrolase; Long chain acyl CoA thioester hydrolase;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q86TX2

ab14592 at 1/100 staining mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14592 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab14592) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....