Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.

Isoform 2 lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.

Isoform 3 lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.

Description
Rabbit polyclonal antibody to Acid sphingomyelinase

Applications 
WB, IF, ICC, IHC

Immunogen 
Acid sphingomyelinase Antibody detects endogenous levels of total Acid sphingomyelinase.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)

Molecular weight
69kDa, 55 kDa; 70kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
Acid sphingomyelinase

Synonyms 
Acid sphingomyelinase; ASM; ASM_HUMAN; aSMase; NPD; Smpd1; Sphingomyelin phosphodiesterase 1 acid lysosomal; Sphingomyelin phosphodiesterase;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P17405
 

Western blot analysis of extracts from HepG2 cells, using Acid sphingomyelinase Antibody. The lane on the left was treated with blocking peptide. Observed bands: 55 kDa....

ab13160 at 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab13160 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab13160 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....

Fig. 2| mRNA and protein levels of acid sphingomyelinase(ASM) in diethylenetriamine/nitric oxide (DETA–NO)-treated HepG2 cells. a mRNA level of ASM in HepG2 cells treated with diferent concentrations of DETA–NO were assayed via real-time polymerase chain reaction. b Protein levels of ASM in HepG2 cells treated with diferent concentrations of DETA–NO were detected via western blotting. *P<0.05, **P<0.01,***P<0.001, compared with untreated HepG2 cells...