Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism. Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA. Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).

Description
Rabbit polyclonal antibody to ACACB

Applications 
WB.

Immunogen 
ACACB Antibody detects endogenous levels of total ACACB.

Reactivity 
Human, Mouse.

Molecular weight
276 kda; 277kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ABCF1

Synonyms 
ACACB; ACACB_HUMAN; ACC-beta; ACC2; ACCB; Acetyl CoA carboxylase beta; Biotin carboxylase; HACC275;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
O00763

Western blot analysis of ACACB using MCF7 whole lysates....