Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.

Description
Rabbit polyclonal antibody to ABCF1

Applications 
WB, IF, ICC, IHC

Immunogen 
ABCF1 Antibody detects endogenous levels of total ABCF1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(91%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(91%)

Molecular weight
95kDa; 96kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
ABCF1

Synonyms 
ABC27; ABC50; ABCF1; ABCF1_HUMAN; ATP binding cassette 50 (TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; ATP binding cassette, sub family F (GCN20), member 1; ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; AU041969; D17Wsu166e; EST123147; GCN20; TNF alpha stimulated ABC protein; TNF-alpha-stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q8NE71

Western blot analysis of extracts from various samples, using ABCF1 Antibody at 1/1000 dilution. Lane 1: Rat brain. Lane 2: Colo205 cells(heat-shock treatment). Lane 3: Mouse testis. Observed bands:100kD....

ab10293 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab10293 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab10293 at 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody....

ab10293 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(ab10293 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue)....