Catalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN.

Description
Rabbit polyclonal antibody to AASDHPPT

Applications 
WB, IF, ICC, IHC

Immunogen 
AASDHPPT Antibody detects endogenous levels of total AASDHPPT.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Molecular weight
36 KD; 36kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
AASDHPPT

Synonyms 
4' phosphopantetheinyl transferase; 4'-phosphopantetheinyl transferase; AASD PPT; AASD-PPT; AASDHPPT; ADPPT_HUMAN; Alpha aminoadipic semialdehyde dehydrogenase phosphopantetheinyl transferase; Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase; CGI 80; HAH P; L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; LYS2; LYS5; LYS5 ortholog;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q9NRN7

Western blot analysis of extracts from Mouse testis, using AASDHPPT Antibody at 1/1000 dilution. Observed bands:25kD....

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using AASDHPPT Antibody at 1/1000 dilution. Observed bands:36kD....

ab14992 at 1/100 staining human gastric cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14992 at 1/100 staining human pancreatic cancer and para-carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....

ab14992 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(#ab14992) and mouse anti-beta tubulin Ab(#T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue)....