Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.

Description
Rabbit polyclonal antibody to A20/TNFAIP3

Applications 
WB, IF, ICC, IHC

Immunogen 
A20/TNFAIP3 Antibody detects endogenous levels of total A20/TNFAIP3.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(90%), Bovine(82%), Horse(82%), Rabbit(82%), Dog(82%)

Molecular weight
90kDa; 90kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
A20/TNFAIP3

Synonyms 
A20; AISBL; MGC104522; MGC138687; MGC138688; OTU domain containing protein 7C; OTU domain-containing protein 7C; OTUD7C; Putative DNA binding protein A20; Putative DNA-binding protein A20; TNAP3_HUMAN; TNF alpha-induced protein 3; TNFA1P2; TNFAIP 3; TNFAIP3 (A20); TNFAIP3; Tumor necrosis factor alpha induced protein 3; Tumor necrosis factor alpha-induced protein 3; Tumor necrosis factor induced protein 3; Tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P21580

Western blot analysis of extracts from Mouse brain, using A20/TNFAIP3 Antibody. The lane on the left was treated with blocking peptide....

Western blot analysis of extracts from various samples, using TNFAIP3 Antibody. Lane 1: Hela treated with blocking peptide; Lane 2: Hela; Lane 3: HepG2....

ab16904 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody....