Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1.

Description
Rabbit polyclonal antibody to 53BP1

Applications 
WB, IF, ICC, IHC

Immunogen 
53BP1 Antibody detects endogenous levels of total 53BP1.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Molecular weight
213kDa; 214kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
53BP1 

Synonyms 
53 BP1; 53BP1; FLJ41424; MGC138366; p202; p53 binding protein 1; p53 BP1; p53-binding protein 1; p53BP1; TP53 BP1; TP53B_HUMAN; Tp53bp1; TRP53 BP1; Tumor protein 53 binding protein 1; Tumor protein p53 binding protein 1; Tumor suppressor p53 binding protein 1; Tumor suppressor p53-binding protein 1;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
Q12888

Western blot analysis of extracts from A431 , using TP53BP1 antibody. The lane on the left was treated with the antigen-specific peptide....

ab17505 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody....

Fig. 5. Olaparib combined with ML216 has a better effect in enhancing total DNA damage. A–D IF staining showing the expression of γH2AX and 53BP1 in PC3 cells treated with ML216 (8 μM) and olaparib (50 μM), ML216 or olaparib monotherapy, or DMSO for 48 h. Scale bars: 25 μm. E Comet assay of PC3 cells treated with ML216 (8 μM) and olaparib (50 μM), ML216 or olaparib monotherapy, or DMSO for 48 h.The olive tail moment was used as an index and was statistically analyzed. Scale bars: 20 μm. F–G Western blot analysis of the expression of proteins involved in the HR and NHEJ pathways in PC3 cells treated with ML216 (8 μM) and olaparib (50 μM), ML216 or olaparib monotherapy, or DMSO for 48 h. Scale bars: 20 μm. Shown are the means ± SD from 3 experiments (*P ...