Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Description
Rabbit polyclonal antibody to 14-3-3 theta

Applications 
WB, IF, ICC, IHC

Immunogen 
14-3-3 theta Antibody detects endogenous levels of total 14-3-3 theta.

Reactivity 
Human, Mouse, Rat.
可预测：Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)

Molecular weight
28 KD; 28kD(Calculated).

Host species 
Rabbit

Ig class 
Immunogen-specific rabbit IgG

Purification 
Antigen affinity purification

Full name 
14-3-3 theta 

Synonyms 
14 3 3 protein T cell; 14 3 3 protein tau; 14 3 3 protein theta; 14 3 3 tau; 14 3 3 theta; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; 1433T_HUMAN; 1C5; HS1; KCIP1; Protein HS1; Protein kinase C inhibitor protein 1; Protein tau; tyr3/trp5 monooxygenase activation protein, theta; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide; Ywhaq;

Storage
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Swissprot 
P27348

 

Western blot analysis of extracts from various samples, using 14-3-3 theta Antibody. Lane 1: Raji cells, blocked with antigen-specific peptides, Lane 2: Raji cells, Lane 3: COLO205 cells, Lane 4: Rat spleen, Lane 5: HepG2 cells....

ab14354 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody....