A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

保存缓冲液
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol 
 

运输条件
2-8°C 
 

靶点 Uniprot 登记号
P01106 
 

靶点基因 ID
4609 
 

靶点全称
Myelocytomatosis oncogene Tag
 

靶点别名
c- Myc epitope Tag 
 

靶点研究领域
Epigenetics and Nuclear Signaling, Cancer, Signal Transduction 
 

亚型
Immunogen-specific rabbit IgG 

Gel: 8%SDS-PAGE, Lysate: 0.4/0.5 μg, Lane 1-2: 0.4/0.5 μg Fusion proteins containing the c-Myc Tag, Primary antibody: D110006(c-Myc Tag Antibody) at dilution 1/5000, Secondary antibody: ab100008(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 40 seconds...