The 6X histidine motif is used as a tag on many recombinant protein to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted protein in transfected or transformed cells. (The background information is obtained from official databases and does not represent the product information.) 

Applications:
WB 
 

Name of antibody:
6X His Tag 
 

Immunogen:
18×His conjugated to KLH
 

Full name:
HHHHHH Tag 
 

WB Predicted band size:
55 kDa 
 

WB Positive control:
Recombinant protein containing the His Tag 
 

WB Recommended dilution:
2000-10000 

Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane: Recombinant protein containing the His Tag, Primary antibody: D110002(6X His Tag Antibody) at dilution 1/4000, Secondary antibody: ab100005(HRP-conjugated Goat anti rabbit IgG) at 1/5000 dilution, Exposure time: 20 seconds...