产品背景

The 6 X histidine motif is used as a Tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-Tag fused to targeted proteins in transfected or transformed cells.

Gel: 8%SDS-PAGE, Lysate: 2 μg, Lane: Fusion proteins containing the His Tag, Primary antibody: ab100001(6×His Tag Antibody) at dilution 1/3000, Secondary antibody: Goat anti mouse IgG at 1/5000 dilution, Exposure time: 1 minute...

The image is immunofluorescence of methanol-fixed 3T3 cells transfected with His-TAF4B vector using ab100001(6×His Tag Antibody) at dilution 1/500...

Immunoprecipitation analysis: Lane 1 is Transfected cell lysate(Input), Lane 2 is Transfected cell lysate using ab100001(6×His Tag Antibody) at dilution 1/200, Lane 3 is Normal Mouse IgG(Control)...